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online pcr primers designs tool  (GenScript corporation)

 
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    GenScript corporation online pcr primers designs tool
    Online Pcr Primers Designs Tool, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/online+tool+primer+designer/10__3390_slash_ijms26073429-424-7-12?v=GenScript+corporation
    Average 90 stars, based on 1 article reviews
    online pcr primers designs tool - by Bioz Stars, 2026-06
    90/100 stars

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    The LAMP primer used in the present study.

    Journal: Frontiers in Plant Science

    Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method

    doi: 10.3389/fpls.2024.1485884

    Figure Lengend Snippet: The LAMP primer used in the present study.

    Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Online NEB LAMP primer design tool ( https://lamp.neb.com/#!/ ).

    Techniques: Sequencing

    Selection of the LAMP primers for Fol one-step LAMP CRISPR/Cas12b detection system. The selection results of sixteen groups LAMP primer were shown from (A–P) respectively. Fol_SIXI_LAMP5 (E) exhibited a better performance than that of others.

    Journal: Frontiers in Plant Science

    Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method

    doi: 10.3389/fpls.2024.1485884

    Figure Lengend Snippet: Selection of the LAMP primers for Fol one-step LAMP CRISPR/Cas12b detection system. The selection results of sixteen groups LAMP primer were shown from (A–P) respectively. Fol_SIXI_LAMP5 (E) exhibited a better performance than that of others.

    Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Online NEB LAMP primer design tool ( https://lamp.neb.com/#!/ ).

    Techniques: Selection, CRISPR

    Validation of the practical samples. Representative images of Fol-infected AMK for 5 months from (A) Pot cultivation experiments and (B) field experiments. (C) Amplification profiles of SIX1 of the practical samples from Pot cultivation experiments and field experiments by using Fol one-step LAMP-CRISPR/Cas12b method, the. The recombinant plasmid was served as the positive control (PC), while the nuclease-free water used for NTC. (D) Amplification profiles of SIX1 of the practical samples from Pot cultivation experiments and field experiments by using qPCR assays. (E) Anneal derivative of SIX1 amplicons within qPCR assays, with an average anneal derivative of 78°C.

    Journal: Frontiers in Plant Science

    Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method

    doi: 10.3389/fpls.2024.1485884

    Figure Lengend Snippet: Validation of the practical samples. Representative images of Fol-infected AMK for 5 months from (A) Pot cultivation experiments and (B) field experiments. (C) Amplification profiles of SIX1 of the practical samples from Pot cultivation experiments and field experiments by using Fol one-step LAMP-CRISPR/Cas12b method, the. The recombinant plasmid was served as the positive control (PC), while the nuclease-free water used for NTC. (D) Amplification profiles of SIX1 of the practical samples from Pot cultivation experiments and field experiments by using qPCR assays. (E) Anneal derivative of SIX1 amplicons within qPCR assays, with an average anneal derivative of 78°C.

    Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Online NEB LAMP primer design tool ( https://lamp.neb.com/#!/ ).

    Techniques: Infection, Amplification, CRISPR, Recombinant, Plasmid Preparation, Positive Control

    The LAMP primer used in the present study

    Journal: Microbiology Spectrum

    Article Title: One-tube detection of Salmonella Typhimurium using LAMP and CRISPR-Cas12b

    doi: 10.1128/spectrum.01271-24

    Figure Lengend Snippet: The LAMP primer used in the present study

    Article Snippet: The Online NEB LAMP primer design tool ( https://lamp.neb.com/ #!/) was used to design the LAMP primer set targeting the ST gene STM4497 (Gene ID: 1256023).

    Techniques: Sequencing